Table 1. Perkinsus spp. and Perkinsus marinus strains used in the study.
Perkinsus sp.* | Strain | ATCC # | Location/year of isolation | Host | Reference |
P. marinus | C13-11 [MA-2-11] | 50896 | Cotuit, MA (USA)/1998 | Crassostrea virginica | |
LICT-1 [CT-1] | 50508 | Long Island Sound, CT (USA)/1998 | Crassostrea virginica | ||
DBNJ-1 [NJ-1] | 50509 | Delaware Bay, NJ (USA)/1993 | Crassostrea virginica | ||
CB5D4 | PRA240 | Bennett Point, MD (USA)/2008 | Crassostrea virginica | [23] | |
CB5D4 | PRA393 | GFP mutant derived from PRA240 | Crassostrea virginica | [13], [23] | |
HCedar2 | 50757 | Cedar Keys, FL (USA)/1998 | Crassostrea virginica | ||
HTtP14 [FL-6] | 50763 | Fort Pierce, FL (USA) | Crassostrea virginica | ||
TXsc | 50983 | Galveston Bay, TX (USA)/1993 | Crassostrea virginica | [28] | |
P. chesapeaki ( = andrewsi) | A8-4a | 50807 | Fox Point, MD (USA)/2001 | Macoma balthica | [25] |
P. olseni ( = atlanticus) | ALG1 | 50984 | Ria Formosa, Algarve (Portugal)/2002 | Tapes decussatus | [24] |
P. mediterraneus | G2 | PRA238 | Menorca (Spain)/2003 | Ostrea edulis | [47] |
P. honshuensis | Mie-3G/H8 | PRA177 | Gokasho Bay, Mie Pref. (Japan)/2002 | Venerupis philippinarum | [56] |
Perkinsus marinus PRA240 was used for the primary screen. A total of eight P. marinus strains isolated from oysters from the East and Gulf Coast of the USA and five Perkinsus spp. from around the world were used for the secondary screen. In all the cases cultures were maintained in Dulbecco modified Eagle's: Ham's F12 (1∶2) supplemented with 5% fetal bovine serum.