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. 2014 Oct 22;9(10):e111051. doi: 10.1371/journal.pone.0111051

Table 1. Perkinsus spp. and Perkinsus marinus strains used in the study.

Perkinsus sp.* Strain ATCC # Location/year of isolation Host Reference
P. marinus C13-11 [MA-2-11] 50896 Cotuit, MA (USA)/1998 Crassostrea virginica
LICT-1 [CT-1] 50508 Long Island Sound, CT (USA)/1998 Crassostrea virginica
DBNJ-1 [NJ-1] 50509 Delaware Bay, NJ (USA)/1993 Crassostrea virginica
CB5D4 PRA240 Bennett Point, MD (USA)/2008 Crassostrea virginica [23]
CB5D4 PRA393 GFP mutant derived from PRA240 Crassostrea virginica [13], [23]
HCedar2 50757 Cedar Keys, FL (USA)/1998 Crassostrea virginica
HTtP14 [FL-6] 50763 Fort Pierce, FL (USA) Crassostrea virginica
TXsc 50983 Galveston Bay, TX (USA)/1993 Crassostrea virginica [28]
P. chesapeaki ( = andrewsi) A8-4a 50807 Fox Point, MD (USA)/2001 Macoma balthica [25]
P. olseni ( = atlanticus) ALG1 50984 Ria Formosa, Algarve (Portugal)/2002 Tapes decussatus [24]
P. mediterraneus G2 PRA238 Menorca (Spain)/2003 Ostrea edulis [47]
P. honshuensis Mie-3G/H8 PRA177 Gokasho Bay, Mie Pref. (Japan)/2002 Venerupis philippinarum [56]

Perkinsus marinus PRA240 was used for the primary screen. A total of eight P. marinus strains isolated from oysters from the East and Gulf Coast of the USA and five Perkinsus spp. from around the world were used for the secondary screen. In all the cases cultures were maintained in Dulbecco modified Eagle's: Ham's F12 (1∶2) supplemented with 5% fetal bovine serum.

*Perkinsus qugwadi and Perkinsus beihaiensis have never been available in culture [57], [58].