Figure 6. Keratinocyte-conditioned medium mediate keratinocyte migration (in vitro wound closure assay).

HaCaT cells were grown to confluence on 24-well tissue culture plates. Then each well was denuded by half from cells and re-coated with fibronectin. The cells were incubated with KCM from DFUs, 0 h. (6A); KCM, 48 h. (6B); KCM with goat polyclonal anti-HBD-2, 48 h (6C); KCM with goat polyclonal anti-LL-37 (6D). Migrations were photographed and monitored for up to 48 h. Data shown are representative of three independent experiments. Bar = 200 µm.