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. 2014 Oct 22;9(10):e111089. doi: 10.1371/journal.pone.0111089

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© 2014 Phadke et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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gpa3Δ and crg1Δ mutations, and overexpression of MAT2, ZNF2, or CPR2 all enhanced self-fertility of 97/433. (A) Two independent gpa3Δ and crg1Δ mutants were analyzed (see also Figure S2). Solo-cultures or (B) confrontation assays with a partner of the a mating type (YL4a on the left side of each panel) were incubated on MS agar for 3 weeks at room temperature (24°C) in the dark and photographed. Scale bar = 50 µm. (C) Mutants were analyzed for elevated expression of the MFα pheromone gene as compared to the wildtype after 48 hours incubation on MS medium (C).