Figure 6. Retention of mitochondria in prkaa1^−/− erythrocytes. (A) Representative electron micrographs of WT and prkaa1^−/− erythrocytes (Scale bars: 500 nm). Arrows: mitochondrial remnants. (B) Erythrocytes containing remnants of mitochondria and other organelles were quantified, and the percentage of erythrocytes containing fragmented organelles was calculated. (C) Representative electron micrographs of WT and prkaa1^−/− erythrocytes (Scale bars: 100 nm). In WT erythrocytes, the arrow points to an autophagosome; in prkaa1^−/− erythrocytes, the arrows point to mitochondria. (D) The number of mitochondria and membranes in each cell was quantified. (E and F) Mitochondrial proteins (COX4I1 and TOMM20) in WT and prkaa1^−/− TFRC⁺ and GYPA⁺ reticulocytes were analyzed by western blotting (M) and quantified by densitometry and normalized to ACTB. n = 4, *P < 0.05 vs. WT. (G) Percentage of TFRC⁺ and GYPA⁺ reticulocytes that were stained by TMRM, ***P < 0.001 vs. WT.