Figure 3. Absence of NPR1 and SA responses leads to a decrease in the accumulation of ubiquitinated proteins. (A) Immunoblot detection of ubiquitin (Ubq antibody Z0458 from Dako) in 3-, 6- and 9-wk-old WT, npr1, atg2 npr1, and atg2 plants. (B and C) Immunoblot detection of (B) NBR1 and (C) ubiquitin in 7-wk-old WT, npr1, atg2 npr1, and atg2 plants, after 24 h of treatment with mock or 100 μM BTH. Amido black or Coomassie Blue staining of the large subunit of RuBisCO serve as loading controls.