Figure 6. JEV NS1 localizes to EDEM1-positive vesicles. (A) JEV-infected Neuro2a cells, and WT and atg5−/− MEFs (MOI 5, 24 h) were stained with EDEM1 (green) and NS1 (red) antibodies. Color-merged images are shown in the third panel. Panels on the extreme right show magnified view of the region marked with *. Scale bar: 10 µm and 3 µm (extreme right panels). (B) Colocalization of NS1 with EDEM1 in Neuro2a, WT and atg5−/− MEFs (C) Postnuclear supernatants of JEV-infected Neuro2a cells were fractionated on a discontinuous Optiprep gradient. Five fractions were collected from top to bottom of the gradient and probed with antibodies against LC3, EDEM1, LAMP1, NS1, NS3, and NS5.