Figure 8. LC3 is an essential host factor for JEV replication. (A) Neuro2a cells transfected with control nontargeting (NT) or Lc3 (Lc3a and Lc3b), Sel1l and Edem1 siRNA were lysed 48 h post-transfection and probed with corresponding antibodies and loading control, GAPDH. (B) Viral RNA levels as determined by qRT-PCR in siRNA treated Neuro2a cells infected with JEV (MOI 5, 24 h). (C) JEV titers in culture supernatants of cells treated as described in (B). The Student t test was used to calculate P values. *P < 0.05, **P < 0.01.