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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Curr Eye Res. 2014 Apr 21;40(1):30–39. doi: 10.3109/02713683.2014.909497

Figure 4.

Figure 4

Viability of cultured HCjE stored for four days at 4°C, 12 and 24°C was quantified with a microplate fluorometer using a calcein-acetoxymethyl ester/ethidium homodimer 1 (EH-1) Live/Dead kit. Calcein-acetoxymethyl ester is converted to the green fluorescent calcein by living cells, while EH-1 stains the nuclei of dead cells red. The bar charts illustrate the total number of calcein-stained live cells (A) and EH-1-stained dead cells (B). Cultured HCjE not subjected to storage served as control. N=6. Error bars indicate standard deviation. * P<0.05 and *** P<0.001, compared to all other groups; § P<0.05, compared to 4°C and 24°C; §§§ P<0.001 compared to 4°C and 24°C.