Effect of GSE and Res on cell proliferation and apoptosis in 4NQO-induced oral (tongue) tumorigenesis. (A) Representative photographs for BrdU and TUNEL staining from 4NQO, 4NQO+GSE and 4NQO+Res groups are presented at 400X; insets represent further magnification of a part of the photographs. The positive stained cells (brown colored) were quantified for (B) BrdU staining and (C) TUNEL staining in tongue tissues from 4NQO, 4NQO+GSE and 4NQO+Res groups following the procedures described in ‘Materials and methods’. The data shown are mean ± SEM of five individual tongue tissues from each group. *, P<0.001; $, P<0.05; GSE, grape seed extract; Res, resveratrol.