Spatiotemporal aspects of cryolesion formation on fNADH channel. A. Snapshots taken before, during, and after the cryoablation procedure as it was performed in excised rat heart perfused with 37°C Tyrode's solution. Scale bar 2 mm. B. fNADH intensity versus the distance from center of the lesion. The line of interest is shown on the right. Traces from 4 post-cryoablation time points are shown (90 sec, 5 min, 10 min, 15 min). Throughout the development of the lesion, the border region remains stable.