Figure 3. Inhibition of mitochondrial Ca²⁺ uptake by Ru360 diminished CCE induced by CPA.

Inhibition of mitochondrial Ca²⁺ uptake by RU360 impaired CCE. Fibroblasts were loaded with Fura 2AM (2 μM) for 60 min. After loading, the media were changed to calcium free BSS, and 3 min later the calcium measurements were initiated. The mitochondrial Ca²⁺ uptake blocker Ru360 (0, 20 μM) was added 2 min after basal [Ca²⁺]_i measurements, and 4 min later CPA (2 μM) was added. Two min later CaCl₂ (final concentration of 2.5 mM) was added. The top panel shows the tracings taken from 50–51 cells. The bottom panel shows the integration of the [Ca²⁺]_i peak over the 3 min interval after calcium addition (495–720 sec). Data are means ± SEM (n=50–51 cells). Different letters indicate values vary significantly (p<0.05) from the other groups by ANOVA followed by Student Newman Keul’s test.