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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Metab Brain Dis. 2014 Apr 22;29(4):1083–1093. doi: 10.1007/s11011-014-9541-4

Figure 4. t-BHP did not alter CCE induced by CPA.

Figure 4

Fibroblasts were loaded with Fura 2AM (2 μM) for 60 min. After loading, the media were changed to calcium free BSS, and 3 min later the calcium measurements were initiated. CPA (2 μM) was added after 1 min of basal [Ca2+]i measurement, and after an additional 3 min, t-BHP (100 μM) was added. Three min later different concentrations of CaCl2 (final concentrations of 0.625, 1.25 or 2.5 mM) were added. The top panel shows the tracings taken from 44–87 cells. The bottom panel shows the integration of the [Ca2+]i peak over the 3 min interval after calcium addition (380 - 660 sec). Data are means ± SEM (n= 44–87 cells).