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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Metab Brain Dis. 2014 Apr 22;29(4):1083–1093. doi: 10.1007/s11011-014-9541-4

Figure 5. t-BHP but not SIN-1 attenuated the impairment of CCE by CGP37157.

Figure 5

Fibroblasts were loaded with Fura 2AM (2 μM) for 60 min. After loading, the media were changed to calcium free BSS, and 3 min later the calcium measurements were initiated. The CPA (10 μM) was added 1 min after CGP37157 (40 μM), t-BHP (100 μM) or SIN-1 (500 μM) was added 3 min later. After an additional 2 min CaCl2 (final concentration of 2.5 mM) was added. The top panel shows the tracings taken from 21–29 cells. The bottom panel shows the integration of the [Ca2+]i peak over the 5 min interval after CPA (10 μM) or 3 min after calcium addition (495–720 sec). Data are means ± SEM (n=21–29 cells). Different letters indicate values vary significantly (p<0.05) from the other groups by ANOVA followed by Student Newman Keul’s test.