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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Metab Brain Dis. 2014 Apr 22;29(4):1083–1093. doi: 10.1007/s11011-014-9541-4

Figure 6. Inhibition of CPA-induced mitochondrial Ca2+ uptake by RU360 was reversed by SIN-1.

Figure 6

SIN-1 abolished the inhibition of CPA-induced CCE by the mitochondrial Ca2+ uptake inhibitor RU360. Fibroblasts were loaded with Fura 2AM (2 μM) for 60 min. After loading, the media were changed to calcium free BSS, and 3 min later the calcium measurements were initiated. RU360 (0, 20 μM) was added 2 min after SIN-1 (500 μM). After an additional 4 min, CPA (2 μM) was added, and 2 min later CaCl2 (final concentration of 2.5 mM) was added. The top panel shows the tracings taken from 42–51 cells. The bottom panel shows the integration of the [Ca2+]i peak over the 3 min interval after calcium addition (495–660 sec). Data are means ± SEM (n= 42–51 cells). Different letters indicate values vary significantly (p<0.05) from the other groups by ANOVA followed by Student Newman Keul’s test.