Figure 8. In the presence of InsP3, blockade of mitochondrial Ca²⁺ uptake, exaggerated BRCS and subsequent CCE.

In the presence of InsP₃, blockade of mitochondrial Ca²⁺ uptake by Ru360 exaggerated BRCS and subsequent CCE. Fibroblasts were loaded with Fura 2AM (2 μM) for 60 min. After loading, the media were changed to calcium free BSS, and 3 min later the calcium measurements were initiated. Mitochondria Ca²⁺ uptake blocker Ru360 (0, 10, 20 μM) was added after 1 min basal [Ca²⁺]_i measurements, and bombesin (1 μM) was added after 3 min. After additional 3 min, CPA (2 μM) was added, and 3 min later CaCl₂ (final concentration of 2.5 mM) was added. The top panel shows the tracings taken from 74–91 cells. The bottom panel shows the integration of the [Ca²⁺]_i peak over the 2–3 min interval after bombesin or calcium addition. Data are means ± SEM (n=74–91 cells). Different letters indicate values vary significantly (p<0.05) from the other groups by ANOVA followed by Student Newman Keul’s test.