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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Metab Brain Dis. 2014 Apr 22;29(4):1083–1093. doi: 10.1007/s11011-014-9541-4

Figure 9. In the presence of InsP3, impairment of CCE by mitochondrial Ca2+ exporter inhibitor was attenuated by t-BHP.

Figure 9

Fibroblasts were loaded with Fura 2AM (2 μM) for 60 min. After loading, the media were changed to calcium free BSS, and 3 min later the calcium measurements were initiated. CGP37157 (20 μM) was added 1 min after t-BHP (100 μM) treatment, and bombesin (1 μM) was added after 3 min. After an additional 2 min, CPA (2 μM) was added, and 2 min later CaCl2 (final concentration of 2.5 mM) was added. The top panel shows the tracings taken from 69–116 cells. The bottom panel shows the integration of the [Ca2+]i peak over the 3 min interval after bombesin or calcium addition. Data are means ± SEM (n=69–116 cells). Different letters indicate values vary significantly (p<0.05) from the other groups by ANOVA followed by Student Newman Keul’s test.