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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Dev Dyn. 2014 Aug 1;243(11):1391–1400. doi: 10.1002/dvdy.24165

Figure 7. Gene expression in the corneal ectoderm is altered following ablation of Pitx2.

Figure 7

Control and Pitx2-tko eyes were immunostained to detect the expression of FOXC2 at e13.5 (A,E), BARX2 at e13.5 (B,F), and keratin 4 (C,G) and keratin 12 (D,H) at e16.5. FOXC2, BARX2 and keratocan 4 are limited to the conjunctival ectoderm in control eyes but also expressed in the corneal ectoderm in Pitx2tko mutant eyes. Keratocan 12 is expressed in the corneal ectoderm of control eyes but is absent from Pitx2-tko mutant eyes. Note: the FOXC2 signal in the lens is background artifact due to the inherent stickiness of crystalline proteins. Co, corneal ectoderm; Cn, conjunctival ectoderm.