Fig. 1. SDF-1 level increased in blood and did not affect osteoblasts. (A) Timeline of the experimental design of this study. Twelve-week-old C57BL/6 mice (n = 40) were divided into four groups (Sham/PBS group [n = 10]; Sham/AMD3100 group [n = 10]; OVX/PBS group [n = 10]; OVX/AMD3100 group [n = 10]). (B, C) Steady-state homeostasis fold change in levels of SDF-1 was evaluated in mouse plasma and BM supernatants after administration of PBS or AMD3100. AMD3100 induced release of functional SDF-1 to plasma. (D) Expression of Osteocalcin, PTHR1, Osterix, and Runx2 was analyzed by Real-Time PCR from BM of OVX and sham mice. Data represent mean ± SEM (Student’s t-test. n = 4-5 per group). *P ＜ 0.05 compared with AMD3100 treated mice or matched control.
