Fig. 1. Effect of palmitate treatment on intracellular lipid accumulation and apoptosis. (A) Human hepatocarcinoma HepG2 cells were incubated with increasing palmitate concentrations (0.5 mM and 1 mM), for 24 h. Cells treated with 1% (w/v) FFA-free BSA served as controls. Lipid droplet accumulation was visualized by Oil-Red-O staining (Original magnification ×200). (B) Time course of PARP and cleaved caspase-3 levels, after treatment with 1 mM of palmitate, as assessed by Western blot analysis. (C) Levels of cleaved caspase-3 activity, as assessed by ELISA assay. The densitometric analyses from n = 3/group are presented; *P ＜ 0.05.
