Fig. 1. (A) Schematic representation of the full-length FADD and c-FLIP domains. (B) Co-expressions of FADD DED and c-FLIP DEDs are shown by SDS-PAGE. (C) Analysis of FADD and structure-based mutations (E80A, L84A, K169A and Y171A) of c-FLIP DEDs, as revealed by a GST-tag pull-down. (D) Analysis of FADD and structure-based mutations (M120A, L143A and Q148A) of c-FLIP DEDs. (E) c-FLIP mutants (F23G and F114G/L115G) binding to FADD. The protein interactions were detected by Western blot. (F) Input controls of FADD and c-FLIP are shown.