Fig. 4. PER3 3'UTR was directly targeted by miR-103 and miR-103 regulated proliferation and migration of SW480 and HCT-116 cells. (A) The repression of luciferase activity by PER3 3'UTR was dependent on miR-103. Mutated PER3 3'UTR abrogated miR-103 mediated repression luciferase activity. (B) qPCR detected the expression of miR-103 after transfecting with pre-miR-103, pre-scramble, anti-miR-103, or anti- scramble. (C) qPCR detected the expression of PER3 mRNA after transfecting with pre-miR-103, pre-scramble, anti-miR-103, or anti- scramble. (D) Colony formation evaluated the proliferation of SW480 and HCT-116 cells treated with pre-miR-103, pre-scramble, anti-miR-103, or anti- scramble. (E, F) MTT assay measured the proliferation of SW480 and HCT-116 cells treated with pre-miR-103, pre-scramble, anti-miR-103, or anti- scramble. (G, H) Transwell assay measured the migration of SW480 and HCT-116 cells treated with pre-miR-103, pre-scramble, anti-miR-103, or anti- scramble. Data shown are means ± S.D, ^#P ＜ 0.05, ^*P ＜ 0.05, ^**P ＜ 0.01 versus control or pre-scramble.