Fig. 1.

Schematic overview of this study. (A) Scheme of the examinations targeting the transgene. Four kinds of fertilized oocytes were used for injection of the TALEN mRNAs. The first two kinds of oocytes were produced by IVF treatment using fresh or frozen sperm, and then cryopreserved. After thawing the oocytes, microinjection of the TALEN mRNAs was performed. The other two kinds of oocytes were fresh or freeze-thawed oocytes collected from female mice mated with male mice. (B) Scheme of the examinations targeting the endogenous gene. Four kinds of fertilized oocytes were used. Sexually mature or immature female mice were mated with male mice, and then fertilized oocytes were collected and used for injection with or without cryopreservation.