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| Group | Fertilization | Sperm | Fertilized oocytes | Injected | Transferred | Newborns | Analyzed pups | Mutants |
|---|---|---|---|---|---|---|---|---|
| MU (control) | Mating | - | Unfrozen | 56 | 40 | 10 (25.0%) | 10 | 8 (80.0%) |
| MF | Mating | - | Frozen | 52 | 48 | 21 (43.8%) | 21 | 7 (33.3%) |
| IUF | IVF | Unfrozen | Frozen | 89 | 72 | 22 (30.6%) | 20 | 10 (50.0%) |
| IFF | IVF | Frozen | Frozen | 53 | 31 | 6 (19.4%) | 6 | 2 (33.3%) |
