Table 1. Primer and probe sequences for VEGF genotyping.
SNP | Sequence (5′→3′) |
rs1413711 | |
Primer sense | 5′-TGACAATATTCTCCCGGGACC-3′ |
Primer antisense | 5′-AGTGTGACCTTCAGAGGCCC-3′ |
Probe-1 | 5′-CTTCCAAGGCCAGGGGGCA-3′-FLa |
Probe-2 | b5′L640-AGGAGGGGCGGTTCTAGGCAGGCA-3′ |
rs2146323 | |
Primer sense | 5′-AAGCTTAGGGAAGTGCTTCAA-3′ |
Primer antisense | 5′-CTGCGCTGATAGACATCCAT-3′ |
Probe-1 | 5′-TGTAATGCCACTCTTTGGAGCTT-3′-FLa |
Probe-2 | b 5′L640-GAATCAGGCAAGTCCTTCC-3′ |
rs3025033 | |
Primer sense | 5′-AAGACTTTGTGGGGATTTCCTA-3′ |
Primer antisense | 5′-TTGGTTTCACATAGGGCCAA-3′ |
Probe-1 | 5′-AGGGAAGTCCTTGGAGTGTCTCCCC-3′-FLa |
Probe-2 | b 5′L640-CCCCAGCAATGTTCTTGTGGC-3′ |
aFL: Fluorescein; b640: LightCycler-Red 640.