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. 1976 Mar;13(3):949–958. doi: 10.1128/iai.13.3.949-958.1976

Immune mechanism in Pasteurella multocida-infected mice.

J B Woolcock, F M Collins
PMCID: PMC420699  PMID: 131783

Abstract

Various immunizing procedures were tested for their effect on the growth of Pasteurella multocida in specific pathogen-free CD-1 mice. Two injections with killed vaccine incorporated into Freund complete adjuvant gave excellent protection against parenteral challenge, but less to control the growth of an aerogenic inoculum. Protection with adjuvant-treated preparation was always superior to that seen with non-adjuvant-treated vaccines. A killed saline-suspended vaccine introduced aerogenically marginally increased resistance to small (2 to 5 mean lethal doses) aerogenic challenges. Live mycobacterium bovis (BCG) vaccine immunopotentiated a single dose of heat-killed P. multocida vaccine in terms of the protective immune response against a subsequent footpad challenge. Hyperimmune mouse serum, administered intraperitoneally, intramuscularly, or intravenously 1 to 7 days prior to subcutaneous challenge with 500 to 5,000 P. multocida, was highly protective. Mice injected in the thigh with 0.2 ml of hyperimmune serum prior to footpad challenge frequently developed a severe inflammatory response with local swelling within 48 h; these lesions often became severly abscessed with time. The passively protected, footpad-infected mice developed active immunity against a subsequent challenge given into the opposite footpad.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alls A. A., Appleton G. S., Ipson J. R. A bird-contact method of challenging turkeys with Pasteurella multocida. Avian Dis. 1970 Feb;14(1):172–178. [PubMed] [Google Scholar]
  2. Carter G. R. Pasteurellosis: Pasteurella multocida and Pasteurella hemolytica. Adv Vet Sci. 1967;11:321–379. [PubMed] [Google Scholar]
  3. Collins F. M. Effect of adjuvant on immunogenicity of a heat-killed salmonella vaccine. J Infect Dis. 1972 Jul;126(1):69–76. doi: 10.1093/infdis/126.1.69. [DOI] [PubMed] [Google Scholar]
  4. Collins F. M. Growth of Pasteurella multocida in vaccinated and normal mice. Infect Immun. 1973 Dec;8(6):868–875. doi: 10.1128/iai.8.6.868-875.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Collins F. M., Mackaness G. B. The relationship of delayed hypersensitivity to acquired antituberculous immunity. I. Tuberculin sensitivity and resistance to reinfection in BCG-vaccinated mice. Cell Immunol. 1970 Sep;1(3):253–265. doi: 10.1016/0008-8749(70)90047-x. [DOI] [PubMed] [Google Scholar]
  6. Collins F. M., Montalbine V. Relative immunogenicity of streptomycin-susceptible and -resistant strains of BCG. II. Effect of the route of inoculation on growth and immunogenicity. Am Rev Respir Dis. 1975 Jan;111(1):43–51. doi: 10.1164/arrd.1975.111.1.43. [DOI] [PubMed] [Google Scholar]
  7. Collins F. M., Scott M. T. Effect of Corynebacterium parvum treatment on the growth of Salmonella enteritidis in mice. Infect Immun. 1974 May;9(5):863–869. doi: 10.1128/iai.9.5.863-869.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Collins F. M. Vaccines and cell-mediated immunity. Bacteriol Rev. 1974 Dec;38(4):371–402. doi: 10.1128/br.38.4.371-402.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hubbert W. T., Rosen M. N. Pasteurella multocida Infections: I. Pasteurella Multocida Infection Due to Animal Bite. Am J Public Health Nations Health. 1970 Jun;60(6):1103–1108. doi: 10.2105/ajph.60.6.1103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lillie L. E., Thomson R. G. The pulmonary clearance of bacteria by calves and mice. Can J Comp Med. 1972 Apr;36(2):129–137. [PMC free article] [PubMed] [Google Scholar]
  11. Miller T. E., Mackaness G. B., Lagrange P. H. Immunopotentiation with BCG. II. Modulation of the response to sheep red blood cells. J Natl Cancer Inst. 1973 Nov;51(5):1669–1676. doi: 10.1093/jnci/51.5.1669. [DOI] [PubMed] [Google Scholar]
  12. Reynolds H. Y., Thompson R. E. Pulmonary host defenses. I. Analysis of protein and lipids in bronchial secretions and antibody responses after vaccination with pseudomonas aeruginosa. J Immunol. 1973 Aug;111(2):358–368. [PubMed] [Google Scholar]
  13. Truitt G. L., Mackaness G. B. Cell-mediated resistance to aerogenic infection of the lung. Am Rev Respir Dis. 1971 Dec;104(6):829–843. doi: 10.1164/arrd.1971.104.6.829. [DOI] [PubMed] [Google Scholar]

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