Table 2.
Many Chs6 mutations have no phenotype.
| Plasmid name | Residues mutated |
|---|---|
| MUTATIONS MADE BEFORE CRYSTAL STRUCTURE | |
| pAS62 | Wild-type Chs6-Myc |
| pJC1 | G540A W541A |
| pJC2 | R548E F552A |
| pJC3 | C582A W585A D587K |
| pAS70 | S237A |
| pAS71 | S253A |
| pAS72 | T516A |
| pAS73 | S612A |
| pAS92 | C216A K217D K218D |
| MUTATIONS BASED ON CRYSTAL STRUCTURE | |
| pAS109 | R713A |
| pAS110 | D724A |
| pAS111 | V728D |
| pAS112 | D731A |
| pAS115 | R713A V728D |
| pAS116 | R713A D731A |
| pAS117 | D724A D731A |
| pAS126 | V728W |
| pAS127 | A735W |
| pAS128 | V728R |
| pAS129 | A735R |
| pAS130 | V728E |
| pAS131 | A735E |
| pAS152 | V728R A735R |
| pAS153 | V728E A735E |
| pAS180 | K210A |
| pAS183 | R637A T638A |
| pAS184 | D672A |
| pAS191 | F34A |
| pAS192 | F34A R713A |
| pAS193 | F34A D731A |
The indicated residues were mutated in a Chs6-Myc plasmid and transformed into a chs6Δstrain. The cells showed no CW resistance. Mutations in italics were made based on the crystal structure in (6A).