Table 3.

Summary of Chs3 mutations analyzed in this study and their effect on trafficking.

Mutant	CW growth phenotype	CW sensitivity rescued by apl2Δ	Localization phenotype	Likely pathways disrupted
chs3Δ	++++	No	N/A	N/A
ΔN52	++++	Strongly	Weak bud neck, few punctae	TGN→PM (exomer) TGN→Endosomes (AP-1,Gga1/2) Endosomes→TGN (Retromer, Snx4/41/42)
19DEE21→AAA	+	Moderately	Normal bud neck and punctae, additional at PM	Endocytosis (AP-2) TGN→Endosomes (AP-1,Gga1/2)
Δ10-27	++	Moderately	Normal bud neck and punctae, additional at PM	Endocytosis (AP-2) TGN→Endosomes (AP-1,Gga1/2)
Δ28-52	+++	No	Weak bud neck, few punctae, vacuole	TGN→PM (exomer) Endosomes→TGN (Retromer, Snx4/41/42)
41LVR43→AAA	++++	Moderately	Weak bud neck, few punctae	TGN→PM (exomer)
ΔC21	++++	No	TGN punctae only (Rockenbauch et al., 2012)	TGN→PM (exomer)