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. Author manuscript; available in PMC: 2014 Oct 23.
Published in final edited form as: Genes Cells. 2014 Jan 23;19(3):209–224. doi: 10.1111/gtc.12125

Figure 2.

Figure 2

Photoactivation analysis of SOD1-G85R-mPAGFP during disappearance of inclusions, in inclusions (A) and in the cytosol (B). Red images in the far left column show the distribution of cotransfected SOD1-G85R-TagRFP. Pseudo-colored images indicate the intensity of photoactivated green fluorescence from SOD1-G85R-mPAGFP. White circles show photoactivated regions. White arrowheads indicate inclusions. Bar = 10 mm. Graphs show quantified fluorescence intensities after photoactivation. The relative cytosolic fluorescence intensity during chase periods (3 and 6 h) was normalized against the intensity immediately after photoactivation (0 h; n = 3–4).