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. Author manuscript; available in PMC: 2014 Oct 23.
Published in final edited form as: J Neurol Neurophysiol. 2014 May;5(3):209. doi: 10.4172/2155-9562.1000209

Figure 6.

Figure 6

Immunohistochemistry and blood-brain barrier analysis in 6-OHDA lesioned RNU−/− rats. Laser-scanning confocal microscopy images of triple immunostained tissues using antibodies against GFAP (red), AQP4 (blue) and laminin (green) indicate BBB changes between the contralateral (A and B) and 6-OHDA lesioned brain (C and D) in RNU−/− rats. Localized morphological and protein expression changes suggests differences in the interaction between astrocytes and blood vessels 4 weeks post 6-OHDA lesion between the contralateral and ipsilateral side in RNU−/−. Magnification at 100× indicated direct association of astrocytic end-feet on blood vessels (A and B). However, AQP4 immunoreactivity was diminished and not localized to blood vessels (laminin/green) in the lesioned tissue (C and D).