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. 2014 Oct 21;7:156. doi: 10.1186/s13068-014-0156-0

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© Hu and Lidstrom; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Ethylmalonyl-CoA pathway in M. extorquens showing intermediates for 1-butanol production. PhaA, β-ketothiolase; PhaB, acetoacetyl-CoA reductase; CroR, crotonase; Ccr, crotonyl-CoA carboxylase/reductase; Ter, trans-2-enoyl-CoA reductase; AdhE2, bifunctional aldehyde/alcohol dehydrogenase. Gray lines represent methylotrophic pathways used exclusively in C1 assimilation. Dashed lines represent the heterologous pathway for 1-butanol production.