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In vitro 13 C-based metabolic flux analysis of wild-type and BHB7 strain. Results are ratios of peak volumes in BHB7 cell extracts compared to wild type. The extracts of ethylamine-grown cells were fed with 13C-labeled acetyl-CoA and radiolabeled metabolites were measured at different reaction times: 5 s (blue), 30 s (black), and 5 min (red).
