Table 1.
M. extorquens strains and plasmids used in this study
| Strain | Plasmid/Genotype * | Reference |
|---|---|---|
| Wild type | Rif derivative | 27 |
| BHB1 | pCM66 (Plac:: bcd&etf-adhE2) | This study |
| BHB2 | pCM80 (PmxaF:: bcd&etf-adhE2) | This study |
| BHB3 | pAP775 (Pmeta1_3616 :: bcd&etf-adhE2) | This study |
| BHB4 | pCM80 (PmaxF:: adhE2-ter) | This study |
| BHB5 | pCM66 (Plac:: adhE2-ter) | This study |
| BHB6 | pAP776 (Pmeta1_002:: adhE2-ter) | This study |
| BHB7 | pAP775 (Pmeta1_3616 :: adhE2-ter) | This study |
| BHB8 | pAP775 (Pmeta1_3616 :: ter-adhE2) | This study |
| BHB9 | pHC61 (Pmtac:: croR:: Pmeta1_3616 :: adhE2-ter) | This study |
| Plasmids | Description | Reference |
| pCM66 | M. extorquens expression vector (Plac, KmR) | 25 |
| pCM80 | M. extorquens expression vector (PmxaF, TcR) | 25 |
| pAP775 | M. extorquens expression vector (Pmeta1_3616, TcR) | E. Skovran |
| pAP776 | M. extorquens expression vector (Pmeta1_002, TcR) | E. Skovran |
| pHC61 | M. extorquens expression vector (Pmtac, KmR) | 47 |
*Promoter strength: PmxaF > Pmeta1_3616 > Pmeta1_002 > Plac.