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. 2014 Oct 17;45(1):99. doi: 10.1186/s13567-014-0099-2

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© Indikova et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Gliding paths of motile M. gallisepticum. A stack of phase-contrast pictures of M. gallisepticum RCL1, captured at 1-s intervals, was manipulated with Fiji by applying a Z projection method [24] to visualize the paths of gliding mycoplasmas. Differential colouring of mycoplasmas (red) and their paths (green) was done with Adobe Photoshop. This qualitative microcinematography motility assay allowed the rapid assessment of the motility of wild-type and mutant strains.