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. 2014 Oct 17;45(1):99. doi: 10.1186/s13567-014-0099-2

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© Indikova et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Quantitative analysis of gliding motility. Time-lapse cinematography was performed for cultures of parental strain RCL1 and the gliding-deficient transposon mutants (T932A, T932C) and mgc2-complemented mutants (T932A::p5Hmgc, T932C::p5Hmgc). Using the image processing Fiji package, movies were analyzed to determine the gliding distance (A) and the average speed (B) of mycoplasmas. The mean values from three independent experiments are shown. Statistically significant differences to RCL1 (P ≤ 0.05) are marked by asterisks.