Figure 1.

Hyperinsulinemic-euglycemic clamps in C57BL/6J mice after 8 weeks of dietary MR. The clamp procedures were conducted as described in Research Design and Methods, and the clamp procedure was initiated at 0 min with a continuous insulin infusion (2.5 mU/kg/min) that was maintained for 140 min. Glucose was infused at a rate sufficient to maintain euglycemia until a steady-state GIR was reached. A: Blood glucose levels during the clamp procedure. B: The GIR required to maintain euglycemia during the insulin clamps. BW, body weight. C: Basal endo R_a and its suppression by insulin during the clamp procedure. D and E: R_g, an indication of tissue-specific insulin-dependent glucose uptake. Means ± SEM are presented for each variable and based on n = 8 for control diet and n = 9 for dietary MR. *Means differ from their corresponding controls at P < 0.05.