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. 2014 Oct 13;63(11):3721–3733. doi: 10.2337/db14-0464

Figure 2.

Figure 2

Short-term signaling responses in liver, muscle, and BAT after an acute injection of insulin in mice after 8 weeks of dietary MR. Mice fed the control or MR diets received intraperitoneal injections of saline (vehicle) or insulin (0.8 units/kg body weight) at time 0. Tissues were harvested after 12 min from the animals in each dietary group and snap frozen. Insulin-dependent phosphorylation (p) of Akt (A), IR (B), activity of IRS1-bound PI3K (C), and increases in PIP3 in liver (D). Insulin-dependent phosphorylation of Akt in gastrocnemius muscle (E) and BAT (F). Total Akt (A, E, and F) was measured as a loading control, and scanning densitometry was used to quantitate expression levels for each protein. Phosphorylated forms of each protein are expressed relative to the total. Means ± SEM are presented for each variable and based on n = 6 for control diet and n = 8 for MR. Means denoted with a different letter (a, b, c, d) differ at P < 0.05.