Table 1.

Changes in body weight, adiposity, liver weight, energy intake, and energy expenditure in C57BL/6J mice after 8 weeks of dietary MR

	Control diet	MR diet
Body weight (g)1	30.4 ± 1.5a	23.8 ± 0.75b
% Fat ([g fat/g BW] × 100)1	23.6 ± 1.96a	15.4 ± 0.57b
% Lean ([g lean/g BW] × 100)1	62.7 ± 1.81a	69.4 ± 0.42b
EWAT (g)1	1.26 ± 0.18a	0.60 ± 0.05b
Liver weight (g)1	0.99 ± 0.06a	0.79 ± 0.05b
Energy
Intake (kJ/day/g BW)	2.19 ± 0.05a	2.77 ± 0.04b
Expenditure (kJ/mouse/h)2	1.89 ± 0.02a	2.37 ± 0.02

BW, body weight.

Means ± SEM from 8 mice per group were compared using t tests.

^a,bMeans differ at P < 0.05.

¹BW, body composition, EWAT, and liver weight were measured after 8 weeks of dietary MR. Body composition was measured by nuclear magnetic resonance, and the % fat and % lean were expressed as a percentage of BW. EWAT and liver weights were measured after euthanasia, and energy intake was the average daily consumption during the course of the study.

²Energy expenditure was measured in a TSE Systems Indirect Calorimetry at the end of the study. All mice were acclimated to chambers for 24 h, followed by continuous recording of O₂ consumption, CO₂ production, energy intake, and activity for 3 days at 40-min intervals. BW and composition were measured before mice entered the chambers and immediately upon exit. The energy expenditure for each mouse at each 40-min interval was calculated as previously described (7). Diet-induced differences in energy expenditure (kJ/mouse/h) were tested using ANCOVA, calculating least squares means that accounted for variation in the energy expenditure attributable to differences in lean mass, fat mass, activity, and energy intake among the mice. The least square means ± SEM for each diet were compared using t tests.