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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Nat Nanotechnol. 2014 May 11;9(8):648–655. doi: 10.1038/nnano.2014.84

Figure 4.

Figure 4

7Cl mediated mRNA silencing modifies endothelial function in vivo. (A) Primary lewis lung Carcinoma (LLC) growth following three 1.0 mg/kg treatments with PBS, siCntrol. siVEGFR-l, or siDII4 (N=7 to 10 animals per group, data shown as average +/− SEM). (B,C) Representative image and quantification of cleaved caspase 3 (CC3) staining, a marker for apoptosis, following treatment with PBS, siCntrol, siVEGFR-l, siDlI4. Normalized CC3+ area defined as the total CC3+ surface area divided by the tumor surface area. Scale bar = 100 μm. (D) Number of pulmonary surface metastases following four 1.0 mg/kg injections with PBS, siCntrol. siVEGFR-l, or siDli4 (N=4 to 6 per group, data shown as average +/− SEM). To measure effects independent of primary tumor growth, animals were not treated until after primary tumor resection. (E) Murine lungs with metastatic lesion removed after treatment with PBS, siluc, siVEGFR-l, or siDlI4 . *p < 0.05, **p<0.002, ***p<0.00l.