Figure 4.

Knockdown of PPARγ by siRNA inhibits differentiation of 3T3-L1 preadipocytes and induces apoptosis during adipogenesis. (a) Effects of PPARγ knockdown was assessed by Oil red-O staining and reverse transcriptase-PCR. NC, Negative control, siPPARγ, PPARγ-knockdown. (b) Cell numbers were determined on days 0 and 2 after differentiation. **P<0.01. Data represent the mean±S.D. (c) Cells with negative control (NC) and knockdown of PPARγ (siPPARγ) were fixed 48 h after induction and then stained with DAPI (4,6-diamidino-2-phenylindole). They were analyzed by fluorescence microscopy or for DNA fragmentation by TUNEL. (d) Protein expression of the adipogenesis-associated targets C/EBPβ, C/EBPα, and PPARγ and apoptosis-related genes caspase 3, Bax, p27, and p21. (e) 3T3-L1 cells stably expressing control vector or G0s2-FLAG were transfected with siPPARγ, and effects on protein expression were examined