Table 1. Antibodies used.
Antibody | Conjugation | Clone | Company |
---|---|---|---|
CD9 | Biotin | KMC8 | BD Biosciences |
CD31 (PECAM-1) | PE/Biotin | MEC 13.3 | BD Biosciences |
CD41 | PE | MWReg30 | BD Biosciences |
CD45 | APCa | 30-F11 | BD Biosciences |
CD71 | PE | R17217 | e-Bioscienceb |
c-Kit/CD117 | APC | 2B8 | BD Biosciences |
KDR/Flk1 | PE | Avas 12α1 | BD Biosciences |
F4/80 | APC | BM8 | e-Bioscience |
Mac1/CD11b | PE | M1/70 | BD Biosciences |
TER119 | Biotin/PerCP-Cy5.5c | TER-119 | BioLegendd |
Tie2/TEK | Biotin | TEK4 | e-Bioscience |
Gr1 | Biotin | RB6-8C5 | BD Biosciences |
Streptavidin | PE/APC/PE-Cy7e | BioLegend | |
Anti-mouse vWF | Purified | Rabbit | Chemiconf |
Anti-mouse IgG1 | Purified | Rabbit | Chemicon |
Anti-rabbit | Alexa-546 | Goat | Invitrogen |
APC: Allophycocyanin
e-Bioscience, Hatfield, UK
PerCP-Cy5.5: PerCP-Cyanin5.5
BioLegend, San Diego, CA, USA
Cy7: Cyanin7
Chemicon International Inc., Temecula, CA, USA