Figure 1.

Representative FACS plot characterizing our newly formed stem-like cells 8 days post-initial TMZ therapy in four human-derived as well as established glioma cell lines. (a) There is a consistent and significant increase of classical CSC markers (CD133, CD15, Sox2, and Oct4) post-TMZ therapy. These results are extendable to at least three out of four glioma cell lines and xenografted specimens studied, except Oct4, which is overexpressed in two out four cell lines and xenografts. (b) The expression of double-positive markers has been selected in order to allow a more reliable categorization. There was a significant increase in GSCs co-expressing both CD133 and CD15 markers. This increase is also observed for the co-expression of CD133 and Sox-2 and CD133 and Oct4. This last combination was not significantly increased in GBM43 xenografts (P: ns). The control group (CTRL) received DMSO. Error bars denote S.E.M. P: ns (P>0.05), *P<0.05, **P<0.01, ***P<0.001, one-way analysis of variance