Figure 2. Presence of Rpb7 in polysomes or in the cytoplasm is not affected by Not5.

A. Wild-type or mutant cells expressing Tap-tagged Rpb7 as indicated were analyzed on sucrose gradients as in Fig. 1C. The polysome profiles and protein loading for these experiments are available in Fig. S15. B. Wild-type and not5Δ cells expressing Rpb7-TT were grown exponentially and stained with anti-CBP antibodies or DAPI as for Fig. 1G. The merged pictures are displayed.