Figure 8.

(a) Fitting probability as a function of number of states with HaMMy (maximum likelihood, ML) and vbFRET (Maximum evidence, ME). Five-state is picked based on the hairpin unfolding model explained in the text. (b) Average FRET efficiencies vs the number of unpaired bases between the donor dye and the acceptor dye predicted by wormlike chain model assuming 0.8, 1.2, and 1.5 nm persistence length of the ssDNA. The curves represent the simulated data (see Experimental Section). The blue dots are the states identified by HaMMy, and the magenta squares are the states identified by vbFRET where the first two points are combined together.