(A) C-14 radiolabeled
Py-Im polyamide 1, targeted
to the DNA sequence 5′-WGWWCW-3′. (B) Compound levels
of 1 in LNCaP and A549 tumor xenografts, compared against
major host organs (kidney, liver, lung). Statistical comparison performed
against the LNCaP tumor concentration of 1. (C) Calibration
of the dual xenograft experiment against the respective single-tumor
versions. All injections were performed intraperitoneally at 20 nmol
per animal (NSG male mouse, N = 10) and tissues harvested
24 h following administration. Each data point represents an individual
organ/tumor analyzed.