Figure 2.

Inhibition of chemokine-stimulated [³⁵S]GTPγS binding by compound 7. HEK293 cells stably expressing CXCR2 were incubated with different concentrations of compound (from 10^–10 to 10^–4 M) at 37 °C for 60 min, and then the membranes were prepared by lysis and centrifugation. The membranes were then incubated in buffer containing 50 μM GDP, 8 nM [³⁵S]GTPγS, and 10 nM CXCL8 at 30 °C for another 60 min. Membranes were harvested by rapid filtration and membrane-bound [³⁵S]GTPγS quantitated. Data, expressed as a percentage of basal [³⁵S]GTPγS bound in the absence of CXCL8, are the mean ± SE from three independent experiments, each done in triplicate.