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Effect of Compound 7 on CXCR2-induced ERK1/2 phosphorylation. HEK293 cells stably expressing CXCR2 were stimulated with CXCL8 (100 ng/mL) for 0–30 min in the presence of vehicle or 10 μM compound 7. Phosphorylated ERK1/2 and total ERK were determined by Western blotting using antiphospho-ERK1/2 (P-ERK1/2) and antitotal ERK1/2 (T-ERK1/2) antibodies, respectively. Data are representative of three independent experiments performed in triplicate.
