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. 2014 Oct 17;8:1965–1977. doi: 10.2147/DDDT.S64989

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© 2014 Wang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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ASP-A induces apoptosis in BEL-7402 cells and inhibits DNA topoisomerase II activity.

Notes: Cells were treated with indicated concentrations of ASP-A for 12 hours, then lysed, separated by SDS-PAGE on 10% gels, and transfered to nitrocellulose membranes. Membranes were immunoblotted with the indicated antibodies, then visualized and quantitated by chemiluminescence detection. (A) Expression of cleaved-caspase-3, cleaved-caspase-8, cleaved-caspase-9, and cleaved-PARP protein in BEL-7402 cells treated with and without ASP-A. (B) Expression of Bax and Bcl-2 protein in BEL-7402 cells treated with and without ASP-A. (C) Protein levels of Bax and Bcl-2 were normalized with β-actin levels and are presented as the ratio of Bax to Bcl-2. Data are presented as mean ± SD, P<0.05. (D) Expression of γ-H2AX in BEL-7402 cells treated with and without ASP-A. (E) Effects of ASP-A and ADM on the decatenation of kDNA by topoisomerase IIα.

Abbreviations: ADM, adriamycin; ASP-A, aspergiolide A; DMSO, dimethyl sulfoxide; kDNA, kinetoplast DNA; PARP, poly(ADP-ribose) polymerase; SD, standard deviation; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gelelectrophoresis; TOPIIα, topoisomerase IIα.