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A – Time dependence of the residual activity of the wild-type and mutant TvDAAOs S67A, S77A, S105A, and S335A at 56°C. 0.1 M potassium phosphate buffer, pH 8.0. Enzyme concentration is 10 μg/ml B – Time dependence of the residual activity of the wild-type and mutant TvDAAOs S277A and S336A at 52°C. 0.1 M potassium phosphate buffer, pH 8.0. Enzyme concentration is 10 μg/ml
