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. 2014 Jul-Sep;6(3):76–88.

Table 1 part 2

D-amino acid	Enzyme
	TvDAAO Ser277Ala			TvDAAO Ser335Ala			TvDAAO Ser336Ala
	k_cat, s^-1	K_M, mM	k_cat/ K_M, mM^-1s^-1	k_cat, s^-1	K_M, mM	k_cat/ K_M, mM^-1s^-1	k_cat, s^-1	K_M, mM	k_cat/ K_M, mM^-1s^-1
D-Met	48.0 ± 1.0	0.73 ± 0.04	65.8	45.9 ± 1.1	4.6 ± 0.2	9.9	56.8 ± 1.5	2.69 ± 0.13	21.1
D-Ala	81.0 ± 3.0	20.4 ± 1.4	4.0	8.2 ± 0.4	10.1 ± 1.2	0.82	101 ± 5.0	24.2 ± 1.9	4.2
D-Ser	no reaction			34.1 ± 1.7	43.0 ± 5.0	0.79	no reaction
D-Val	71.0 ± 3.0	34.0 ± 3.0	2.1	20.1 ± 1.3	25.0 ± 3.0	0.82	50.0 ± 2.0	8.2 ± 0.9	6.1
D-Tyr	0.45 ± 0.06	0.70 ± 0.14	0.65	5.5± 0.9	0.58 ± 0.13	9.5	15.8 ± 0.7	0.36 ± 0.03	44.2
D-Trp	0.49 ± 0.04	0.26 ± 0.02	1.9	79.0 ± 5.0	1.23 ± 0.11	64.1	57.0 ± 1.0	0.69 ± 0.04	82.0
D-Leu	12.0 ± 2.0	0.15 ± 0.02	80.0	5.9 ± 0.1	0.21 ± 0.01	27.5	5.0 ±0.1	0.07 ± 0.01	76.4
D-Phe	15.1 ± 0.4	0.18 ± 0.03	83.9	8.3 ± 0.2	0.38 ± 0.03	21.9	11.8 ± 0.3	0.24 ± 0.02	49.4
D-Asn	11.8 ± 1.2	8.0 ± 1.5	1.48	46.0 ± 4.0	26.0 ± 3.0	1.78	32.0 ± 2.0	16.0 ± 3.0	2.0
D-Thr	no reaction			1.8 ± 0.1	14.0 ± 2.0	0.13	no reaction
D-Lys	no reaction			no reaction			no reaction

^* Improved catalytic parameters of mutant TvDAAOs in comparison to wild-type are marked with bold font and gray background