Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Oct 23;10(10):e1004650. doi: 10.1371/journal.pgen.1004650

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Hughes, Hawley

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

DNA is labeled with DAPI (blue), heterochromatin is labeled with an antibody recognizing histone 3 trimethylated on lysine 9 (H3K9me3) (green), and the spindle is labeled with an antibody recognizing α-tubulin (red). (A) Top2 RNAi/+ control oocyte with achiasmate 4^th chromosomes that have moved towards the spindle poles. The 4^th chromosomes and the centromeric regions of the chromosomes are labeled with H3K9me3 and are oriented towards opposite spindle poles. (B–C) Top2 RNAi/matαGAL oocytes containing abnormal DNA projections that are labeled with the H3K9me3 antibody. Additionally, the H3K9me3 antibody localization in the chromosome mass of both oocytes is not oriented towards opposite spindle poles. Images are projections of partial Z-stacks. Scale bars are 5 microns.